ELSI

研究・活動

研究者

塚谷 祐介

塚谷 祐介
氏 名
塚谷 祐介
役 職
研究員
専門分野
生化学・分子生物学
E-mail
tsukatani_at_elsi.jp

主要業績/受賞歴

< Research Article >

34. Kasuno, M., Kimura, H., Yasutomo, H., Torimura, M., Murakami, D., Tsukatani, Y., Hanada, S., Matsushita, T., and Tao, H. (2016) Evaluation of sensor performance for harmful compounds by using photo-induced electron transfer from photosynthetic membranes to electrodes. Sensors, 16, pii: E438.
DOI: 10.3390/s16040438.

33. Tsukatani, Y., Mizoguchi, T., Thweatt, J., Tank, M., Bryant, D.A., and Tamiaki, H. (2016) Glycolipid analyses in envelope protein mutants of light-harvesting chlorosomes of Chlorobaculum tepidum. Photosynth. Res. 128, 235-241.

32. Harada, J., Teramura, M., Mizoguchi, T., Tsukatani, Y., Yamamoto, K., and Tamiaki, H. (2015) Stereochemical conversion of C3-vinyl group to 1-hydroxyethyl group in bacteriochlorophyll c by the hydratases BchF and BchV: adaptation of green sulfur bacteria to limited-light environments. Mol. Microbiol. 98, 1184-1198.

31. Tsukatani, Y., Hirose, Y., Harada, J., Misawa, N., Mori, K., Inoue, K., and Tamiaki, H. (2015) Complete genome sequence of the bacteriochlorophyll b-producing photosynthetic bacterium Blastochloris viridis. Genome Announc. 3, pii: e01006-15.
DOI:10.1128/genomeA.01006-15

30. Tsukatani, Y., and Masuda, S. (2015) Elucidation of genetic backgrounds necessary for chlorophyll a biosynthesis toward artificial creation of oxygenic photosynthesis. Orig. Life Evol. Biosph. 45, 367-369. DOI: 10.1007/s11084-015-9446-1

29. Tsukatani, Y., Harada, J., Nomata, J., Yamamoto, H., Fujita, Y., Mizoguchi, T., and Tamiaki, H. (2015) Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways. Sci. Rep. 5, 9741. DOI: 10.1038/srep09741

28. Mizoguchi, T., Harada, J., Tsukatani, Y., and Tamiaki, H. (2015) The 17-propionate esterifying variants of bacteriochlorophyll-a and bacteriopheophytin-a in purple photosynthetic bacteria. J. Photochem. Photobiol. B 142, 244-249. DOI: 10.1016/j.jphotobiol.2014.12.013

27. Harada, J., Mizoguchi, T., Tsukatani, Y., Yokono, M., Tanaka, A., and Tamiaki, H. (2014) Chlorophyllide a oxidoreductase works as one of the divinyl reductases specifically involved in bacteriochlorophyll a biosynthesis. J. Biol. Chem. Vol. 289, 12716-12726.

26. Mizoguchi, T., Harada, J., Tsukatani, Y., and Tamiaki, H. (2014) Isolation and characterization of a new bacteriochlorophyll-c bearing a neopentyl substituent at the 8-position from the bciD-deletion mutant of the brown-colored green sulfur bacterium Chlorobaculum limnaeum. Photosynth. Res. Vol. 121, 3-12.

25. Tsukatani, Y., Harada, J., Mizoguchi, T., and Tamiaki, H. (2013) Bacteriochlorophyll homolog compositions in the bchU mutants of green sulfur bacteria. Photochem. Photobiol. Sci. Vol. 12, 2195-2201.

24. Sadaoka, K., Shoji, S., Hirota, K., Tsukatani, Y., Yoshitomi, T., Tamiaki, H., Kashimura, S., and Saga, S. (2013) Pheophytinization kinetics of chlorophyll c under weakly acidic conditions: effects of acrylic acid residue at the 17-position. Bioorg. Med. Chem. Vol. 21, 6915-6919.

23. Adams, P.G., Cadby, A.J., Robinson, B., Tsukatani, Y., Tank, M., Wen, J., Blankenship, R.E., Bryant, D.A., and Hunter, C.N. (2013) Comparison of the physical characteristics of chlorosomes from three different phyla of green phototrophic bacteria. Biochim. Biophys. Acta Vol. 1827, 1235-1244.

22. Tsukatani, Y., Yamamoto, H., Mizoguchi, T., Fujita Y., and Tamiaki, H. (2013) Completion of biosynthetic pathways for bacteriochlorophyll g in Heliobacterium modesticaldum: The C8-ethylidene group formation. Biochim. Biophys. Acta Vol. 1827, 1200-1204

21. Mizoguchi, T., Tsukatani, Y., Harada, J., Takasaki, S., Yoshitomi, T., and Tamiaki, H. (2013) Cyclopropane-ring formation in the acyl groups of chlorosomal glycolipids is crucial for acid resistance of green bacterial antenna systems. Bioorg. Med. Chem. vol. 21, 3689-3694.

20. Harada, J., Mizoguchi, T., Satoh, S., Tsukatani, Y., Yokono, M., Noguchi, M., Tanaka, A., and Tamiaki, T. (2013) Specific gene bciD for C7-methyl oxidation in bacteriochlorophyll e biosynthesis of brown-colored green sulfur bacteria. PLOS ONE vol. 8, e60026. DOI: 10.1371/journal.pone.0060026

19. Tsukatani, Y., Yamamoto, H., Harada, J., Yoshitomi, T., Nomata, J., Kasahara, M., Mizoguchi, T., Fujita, Y., and Tamiaki, T. (2013) An unexpectedly branched biosynthetic pathway for bacteriochlorophyll b capable of absorbing near-infrared light. Scientific Reports vol. 3, 1217. DOI: 10.1038/srep01217

18. Furumaki, S., Yabiku, Y., Habuchi, S., Tsukatani, Y., Bryant, D.A., and Vacha, M. (2012) Circular dichroism measured on single chlorosomal light harvesting complexes of green photosynthetic bacteria. J. Phys. Chem. Lett. vol. 3, 3545?3549. DOI: 10.1021/jz301671p 

17. Harada, J., Mizoguchi, T., Tsukatani, Y., Noguchi, M., and Tamiaki, H. (2012) A seventh bacterial chlorophyll driving a large light-harvesting antenna. Scientific Reports vol. 2, 671. 
DOI: 10.1038/srep00671

16. Ganapathy, S., Oostergetel, G., Reus, M., Tsukatani, Y., Gomez Maqueo Chew, A., Buda, F., Bryant, D.A., Holzwarth, A.R., and de Groot, H.J. (2012) Structural variability in wild-type and bchQ bchR mutant chlorosomes of the green sulfur bacterium Chlorobaculum tepidum. Biochemistry vol. 51, 4488?4498.

15. Tsukatani, Y., Romberger, S.P., Golbeck, J.H., and Bryant, D.A. (2012) Isolation and characterization of a homodimeric type-I reaction center complex from “Candidatus Chloracidobacterium thermophilum,” an aerobic chlorophototroph. J. Biol. Chem. vol. 287, 5720-5732.

14. Garcia Costas, A.M., Tsukatani, Y., Rijpstra, W.I.C., Schouten, S., Welander, P.V., Summons, R.E., and Bryant, D.A. (2012) Identification of the bacteriochlorophylls, carotenoids, quinones, lipids, and hopanoids of “Candidatus Chloracidobacterium thermophilum.” J. Bacteriol. vol. 194, 1158-1168.

13. Garcia Costas, A.M., Tsukatani, Y., Romberger, S.P., Oostergetel, G.T., Boekema, E.J., Golbeck, J.H., and Bryant, D.A. (2011) Ultrastructural analysis and identification of envelope proteins of “Candidatus Chloracidobacterium thermophilum” chlorosomes. J. Bacteriol. vol. 193, 6701-6711.

12. Furumaki, S., Vacha, F., Habuchi, S., Tsukatani, Y., Bryant, D.A., and Vacha, M. (2011) Absorption linear dichroism measured directly on a single light-harvesting system: The role of disorder in chlorosomes of green photosynthetic bacteria. J. Am. Chem. Soc. vol. 33, 6703-6710.

11. Wen, J., Tsukatani, Y., Cui, W., Zhang, H., Gross, M.L., Bryant, D.A., and Blankenship, R.E. (2011) Structural model and spectroscopic characteristics of the FMO antenna protein from the aerobic chlorophototroph, Candidatus Chloracidobacterium thermophilum. Biochim. Biophys. Acta vol.1807, 157-164.

10. Tsukatani, Y., Wen, J., Blankenship, R.E., and Bryant, D.A. (2010) Characterization of the FMO protein from the aerobic chlorophototroph, Candidatus Chloracidobacterium thermophilum. Photosynth. Res. vol. 104, 201-209.

9. Kasuno, M., Torimura, M., Tsukatani, Y., Murakami, D., Hanada, S., Matsushita, T., and Tao, H. (2009) Characterization of the photoinduced electron transfer reaction from the photosynthetic system in Rhodobacter sphaeroides to an exogenous electron acceptor. J. Electroanal. Chem. vol. 636, 101-106.

8. Tsukatani, Y., Nakayama, N., Shimada, K., Mino, H., Itoh, S., Matsuura, K., Hanada, S., and Nagashima, K. V.P. (2009) Characterization of a blue copper protein, auracyanin, of the filamentous anoxygenic phototrophic bacterium Roseiflexus castenholzii. Arch. Biochem. Biophys. vol. 490, 57-62.  

7. Azai, C., Tsukatani, Y., Harada, J., and Oh-Oka, H. (2009) Sulfur oxidation in mutants of the photosynthetic green sulfur bacterium Chlorobium tepidum devoid of cytochrome c-554 and SoxB. Photosynth. Res. vol. 100, 57-65.

6. Tsukatani, Y., Azai, C., Kondo, T., Itoh, S., and Oh-Oka, H. (2008) Parallel electron donation pathways to cytochrome cz in the type I homodimeric photosynthetic reaction center complex of Chlorobium tepidum. Biochim. Biophys. Acta vol. 1777, 1211-1217.

5. Tsukatani, Y., Miyamoto, R., Itoh, S., and Oh-Oka, H. (2006) Soluble cytochrome c-554, CycA, is not essential for photosynthetic electron transfer in Chlorobium tepidum. FEBS lett. vol. 580, 2191-2194.

4. Tsukatani, Y., Miyamoto, R., Itoh, S., and Oh-Oka, H. (2004) Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation: Regulation of energy transfer and ferredoxin-mediated NADP+ reduction on the cytoplasmic side. J. Biol. Chem. vol. 279, 51122-51130.

3. Alric, J., Tsukatani, Y., Yoshida, M., Matsuura, K., Shimada, K., Hienerwadel, R., Schoepp-Cothenet, B., Nitschke, W., Nagashima, K.V.P., and Vermeglio, A. (2004) Structural and functional characterization of the unusual triheme cytochrome bound to the reaction center of Rhodovulum sulfidophilum. J. Biol. Chem. vol. 279, 26090-26097.

2. Tsukatani, Y., Matsuura, K., Masuda, S., Shimada, K., Hiraishi, A., and Nagashima, K.V.P. (2004) Phylogenetic distribution of unusual triheme to tetraheme cytochrome subunit in the reaction center complex of purple photosynthetic bacteria. Photosynth. Res. vol. 79, 83-91.

1. Masuda, S., Tsukatani, Y., Kimura, Y., Nagashima, K.V.P., Shimada, K., and Matsuura, K. (2002) Mutational analyses of the photosynthetic reaction center-bound triheme cytochrome subunit and cytochrome c2 in the purple bacterium Rhodovulum sulfidophilum. Biochemistry vol. 41, 11211-11217. 3.226/2010

< Review >

6. 浅井智広, 塚谷祐介 (2016) 酸素発生型光合成の成立過程とその実証方法の再考. 光合成研究 26, 43-58.

5. Tamiaki, H., Teramura, M., and Tsukatani, Y. (2016) Reduction processes in biosynthesis of chlorophyll molecules: chemical implication of enzymatically regio- and stereoselective hydrogenations in the late stages of their biosynthetic pathway. Bull. Chem. Soc. Jpn. 89, 161-173.

4. 塚谷祐介,民秋均, 溝口正 (2015) 光合成細菌の脂質.光合成研究 25, 151-159.

3. 塚谷祐介,民秋均 (2015) 近赤外光を吸収するバクテリオクロロフィル色素の生合成経路解明と応用. 生化学 87, 234-238. DOI: 10.14952/SEIKAGAKU.2015.870234

2. Azai, C., Tsukatani, Y., Itoh, S., and Oh-oka, H. (2010) C-type cytochromes in the photosynthetic electron transfer pathways in green sulfur bacteria and heliobacteria. Photosynth. Res. 104, 189-199.

1. 塚谷祐,浅井智広,大岡宏造 (2010) 緑色硫黄細菌の非循環型の光合成電子伝達系.光合成研究 20, 100-108.

< Patent >

4. 国際特許PCT-JP2015-54552、国際公開WO2015/125849
発明の名称: バクテリオクロロフィルbの大量産生方法及び産生菌
出願人: 学校法人立命館、学校法人久留米大学
発明者: 塚谷祐介、民秋均、原田二朗、藤田祐一、野亦次郎

3. 特願2014-030085
発明の名称: バクテリオクロロフィルbの大量産生方法及び産生菌
発明者: 塚谷祐介、民秋均、原田二朗、藤田祐一、野亦次郎

2. 特願2012-141370、特開2014-3938
発明の名称: 新規酵素及びそれを用いたバクテリオクロロフィルの製造方法
発明者: 塚谷祐介、民秋均

1. 特願2008-90349、特開2009-244056
発明の名称: 水中物質検出装置
発明者: 恩田建介、北川政美、中島芳子、鳥村政基、花田智、糟野潤、村上大亮、田尾博明、塚谷祐介