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Tokyo Tech Scientists Discover Deep Microbes' Key Contribution to Earth's Carbon Cycle

New findings highlight the importance of Microorganisms in the geochemistry of natural gas and petroleum.

Hydrocarbons play key roles in atmospheric and biogeochemistry, the energy economy, and climate change. Most hydrocarbons form in anaerobic environments through high temperature or microbial decomposition of organic matter. Subsurface microorganisms can also "eat" hydrocarbons, preventing them from reaching the atmosphere. Using a new technique, Tokyo Tech scientists show that biological hydrocarbon degradation gives a unique biological signature. These findings could help detect subsurface biology and understand the carbon cycle and its impact on climate.


Hydrocarbons play key roles in atmospheric and biogeochemistry, the energy economy, and climate change. Most hydrocarbons form in anaerobic environments through high temperature or microbial decomposition of organic matter. Microorganisms can also "eat" hydrocarbons underground, preventing them from reaching the atmosphere. Using a new technique developed at the Earth-Life Science Institute (ELSI), an international team led by the Tokyo Institute of Technology professors Alexis Gilbert, Naohiro Yoshida and Yuichiro Ueno show that biological hydrocarbon degradation gives a unique biological signature. These findings could help detect subsurface biology and understand the carbon cycle and its impact on climate.

Humanity exploits Earth's vast reservoirs of hydrocarbons as one of its principle energy sources. The ways in which carbon is fixed and processed during the formation of these reservoirs have important consequences for resource exploration. In addition, the release of hydrocarbons from Earth's subsurface reservoirs can have important implications on Earth's climate, since light hydrocarbons such as methane are potent greenhouse gases. Scientists would like to understand the potentially important role Earth's enormous subsurface biosphere might play in deep hydrocarbon reservoir behaviour. To date, it has been difficult to estimate how much hydrocarbons have been affected by subsurface microorganisms.

Gilbert and coworkers overcame this difficulty by using a new method developed at ELSI that enables the measurement of position specific stable carbon isotope ratios. Hydrocarbons are mostly long chains of carbon atoms attached to hydrogen atoms, but carbon has two naturally abundant isotopes (types of carbon atom with different numbers of neutrons, and thus different masses, which can be measured), carbon-12 (12C) and carbon-13 (13C). Due to the ways organisms form the molecules that ultimately become environmental hydrocarbons, the ratio of 12C/13C for each specific carbon atom position in a hydrocarbon can be unique. The research here focused on propane, a natural gas hydrocarbon molecule containing three carbon atoms.

The researchers fed propane to microorganisms in the lab to measure the specific 12C/13C signature produced these organisms, and measured the non-biological changes that occurred when propane is broken down at high temperatures, a process known as "cracking." They then used these baseline measurements to interpret natural gas samples from the US, Canada and Australia, allowing them to detect the presence of microorganisms using propane as "food" in natural gas reservoirs, and to quantify the amount of hydrocarbons eaten by microorganisms. "When I started analyzing samples from the bacterial simulation experiments, they matched perfectly what we observed in the field, suggesting the presence of propane degrading bacteria in the natural gas reservoirs," Gilbert noted. Thus, this study revealed the presence of microorganisms that would have been difficult to detect using conventional methods, and opens a new window to understanding global hydrocarbon cycling.

"I was particularly interested in deciphering biological from non-biological processes related to organic molecules. This question has implication for the origin of life, for detection of life in the Universe, but also for our understanding of the biosphere and its evolution on Earth," says Gilbert. This study also has important implications with global climate change, as propane and other hydrocarbons are greenhouse gases and pollutants. Though the team did not attempt to quantify how much hydrocarbons are being "eaten" by microorganisms at the global scale, they believe their approach will allow such quantification in the near future, and suggest this will benefit models aiming to quantify global hydrocarbon cycling.

Finally, Gilbert adds, in the future this kind of approach may be useful for the detection of life on extraterrestrial bodies such as other planets or moons in our solar system. Though their current machine is too large to be sent to space, their techniques could be applied to samples brought back to Earth, or their instrument could be miniaturized.

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Figure 1:
Caption - Natural gas reservoirs examined in the study. Red symbols indicate reservoirs where biodegradation was detected.
Credit: Alexis Gilbert, Tokyo Tech

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Figure 2:
Caption - Typical samples analyzed in the study. Left-hand side: Incubation of bacteria with propane. Right-hand side: Natural gas sample.
Credit - N. Escanlar/ELSI

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Figure 3:
Caption - Analysis of molecular isotope species of propane of natural gas samples. Arrows indicate data from simulation experiments: cultivation of propane-degrading bacteria (red) and "cracking" (light blue). Red symbols indicate samples that have been degraded by bacteria.
Credit: Alexis Gilbert, Tokyo Tech

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Figure 4:
Caption - Carbon Isotopic molecular species of propane and the different speed at which they are metabolized by bacteria.
Credit: Yuichiro Ueno, ELSI

Reference
Alexis Gilbert1,2, Barbara Sherwood Lollar3, Florin Musat4, Thomas Giunta3, Songcan Chen4, Yuki Kajimoto5, Keita Yamada4, Christopher J. Boreham6, Naohiro Yoshida1,5, and Yuichiro Ueno1,2, Intramolecular Isotopic Evidence for Bacterial Oxidation of Propane in Subsurface Natural Gas Reservoirs, Proceedings of the National Academy of Sciences, DOI: https://doi.org/10.1073/pnas.1817784116

1. Department of Earth and Planetary Sciences, Tokyo Institute of Technology, Meguro, 152-8551 Tokyo, Japan.
2. Earth-Life Science Institute, Tokyo Institute of Technology, Meguro, 152-8550 Tokyo, Japan.
3. Department of Earth Sciences, University of Toronto, Toronto, ON, Canada M5S 3B1.
4. Helmholtz Centre for Environmental Research - UFZ, 04318 Leipzig, Germany.
5. Department of Environmental Chemistry and Engineering, Tokyo Institute of Technology, Yokohama, 226-8503 Kanagawa, Japan.
6. Geoscience Australia, Canberra, ACT 2601, Australia.

Contact
Alexis Gilbert
Assistant Professor, Earth and Planetary Science Department, Tokyo Institute of Technology
Affiliated Scientist, Earth-Life Science Institute (ELSI), Tokyo Institute of Technology
E-mail: gilbert.a.aa@m.titech.ac.jp
Tel: +81-3-5734-2617 Fax: +81-3-5734-3537

PR Office
Earth-Life Science Institute (ELSI), Tokyo Institute of Technology
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